Title : New insight of non-invasive diagnosis in endometriosis
Abstract:
This presentation will discuss alternative methods in determining the diagnosis of endometriosis using non-invasive methods. Detection of endometriosis using immunocytochemistry of P450 Aromatase expressions in eutopic endometrial cells obtained from menstrual sloughing: a diagnostic study can provide a solution and alternative non-invasive method for diagnosing endometriosis by isolating endometrial cells that have stem cell/progenitor properties taken through blood menstruation in endometriosis patients. This research aimed to explore the possibility of a new diagnostic approach of endometriosis based on immunocytochemistry scoring of Aromatase P450 expressions in endometrial cells collected from menstrual sloughing. In this research The P450 Aromatase expression in endometrial cells of women with endometriosis was significantly stronger than without one. The cut-off point of H-scores to detect endometriosis was > 4. By this criteria, H-score had 94.6% sensitivity, 90.9% specificity, 92% positive predictive value and 93% negative predictive value. Immuno- cytochemistry scoring of Aromatase P450 expression in endometrial cells (ICAPEC) derived from menstrual blood specimen was a good candidate as alternatives approach in diagnostic procedure of endometriosis. Application and evaluation in clinical practice would provide the economically benefit in diagnostic procedure. This research can provide solutions to facilitate the diagnosis of endometriosis, its more simple and non-invasif and it gives benefit for patient who has doubt with invasif or surgery procedure.
