2nd Edition of International Conference on Cell & Stem Cell Research

August 21-23, 2023 | London, UK

August 21 -23, 2023 | London, UK
Stem Cells - 2023

Rekha Kumari

Speaker at Cell & Stem Cell Research 2023 - Rekha Kumari
Institute of Liver and Billiary Sciences, India
Title : Optimized protocol for generating clinical grade umbilical cord tissue mesenchymal stem cell for cell therapy in various disease


Mesenchymal stem cell is being used for therapeutic purpose because of their self-renewal, Immunomodulatory capability and mitochondrial transfer signalling. Mesenchymal stem cells can be isolated from various sources eg, Umbilical Cord, Bone Marrow, Adipose Tissue, Dental Pulp and amniotic fluid. Most frequently used is umbilical cord tissue mesenchymal stem cell due to easily available. In this study three different methods and media are evaluated for isolation and expansion and to check regenerative properties in animal model of acute liver injury. Healthy umbilical cord tissue was collected in PBS containing 10%FCS with 2% Antibiotics. Umbilical cord tissue washed and cleaned in Povidone. MSCs are isolated by three different methods, UC Dissociation Kit (Millitenyi), Explant Culture and Collagenase from 1 gram of cord tissue. These isolated MSC were grown in three different medias GMP Basal Brew Media, Stempeutics (Stem cells Technology) and alpha MEM with 10% FCS (Gibco Life Technology). All cells were characterised for morphology, surface marker expression, population doubling time, CFU-F, Bioenergetics. In vivo therapeutic potential of Umbilical Cord MSC compared with adult Bone Marrow MSC s in drug induced liver injury. Dissociation Kit method gave more yield of MSCs (3×10^5) in shortest time as compare to Collagenase (2.3×10^5) and Explant (1.5×10^5). There is no significant difference are observed in Surface Markers present on MSC Surface. MSCs isolated from all three different methods showed similar characteristic surface marker expression of MSCs. There is significant difference were found in Population Doubling Time and CFU as per shown in Graph. MSCs isolated from Dissociation kit have lowest population doubling dime and have increased CFU ability then others Methods. From cultured MSC in three different medias, BREW media provide best culture condition for expansion of MSCs in vitro wrt   clear spindle shape morphology and have more OCR (Oxygen Consumption Rate) by Bioenergetics assay. Mice treated with UC-MSCs showed better therapeutic potential then BM-MSCs in terms of survival and reduction of liver injury. From this study concluded Umbilical Cord Dissociation Kit and Basal Brew media was best among for isolation and expanding clinical grade MSC. MSC isolated from all three methods showed similar characteristic surface marker expression of MSC. MSC from Umbilical Cord Dissociation kit have lowest population doubling time and have increases CFU ability then Mice treated with Umbilical cord tissue MSC shows better therapeutic than Bone Marrow MSC in terms of survival and reduction of liver injury.


Rekha Kumari 5th year Part time PhD Student in department Amity Institute of Molecular Medicine and Stem Cell Research (AIMMSCR), Amity University., Noida from June 2017-till now.  I am  working as a Junior Research Fellow in a Department of Molecular and Cellular Medicine, at Institute of Liver and Biliary Science, New Delhi Duration ( March 2016- till now). In past years. From 2012-2014 workes as a Junior Scientist at Reviva Cell and Research Therapies, Cryobanks India PVT LTD, Gurgaon. Received Master Degree in Biotechnology from Mody Institute of Technology and Sciences, Lakshmangarh, Sikar Rajasthan. I have 4-5 Research Article as a couthor publication during PhD in journal Names- Hepatology International, Americal Journal of Stem Cell, Frontiers Immunology. I have presented free paper in 3rd Internation Conference on stem cells and regenerative medicine(ICSCG) Indian Stem Cell Study Group at DY Patil Vidyapeeth University at pimperi pune, Maharasthra.