Cell culture is a term used to describe laboratory techniques that allow eukaryotic or prokaryotic cells to develop in physiological settings. In the exploration of basic scientific and translational research concerns, cell culture is a very versatile technique. The uniformity of cell lines and the related reproducibility of data obtained are advantages of using them in scientific study. Cell treatment, tissue engineering, and regenerative medicine, as well as pharmacological and biotechnological uses, all hold enormous promise for stem cells (SCs). While many stem cell culture techniques are similar to those used for somatic cell culture, their unique properties necessitate a more specialised approach.